Gene expression at different cell stages of in vitro-fertilized bovine embryos

The birth rate of embryos produced in vitro (IVF) is still lower than that vivo. Three major steps for the success IVF technique are maturation immature oocytes, fertilization matured and culture resulting embryos. Studying mRNA expression early embryonic development stages important can help to assess embryo quality optimize production protocols vitro. current study aimed determine levels developmentally genes different bovine Cumulus-oocyte complexes (COCs) were collected from ovaries cultured synthetic oviduct fluid (SOF) medium 7 - 9 days. Embryos at time-points listed above, involved pluripotency (OCT4), DNA methylation (DNMT1), apoptosis (BAX), metabolism (GLUT1) a heat shock protein (HSP70) was estimated 2-cell stage blastocyst results showed statistically significant differences relative abundance (RA) OCT4, DNMT1, BAX, GLUT1 gene transcripts among stages, whereas there non-significant RA HSP70 between these stages. In conclusion, differ developmental vitro, possibly because timing genome activation (EGA).